Me pēhea e mahi ai ngā Bio-factors Biotherapy hei whakapai ake i te whakapapa kiko

Te mātai i te Biotherapy me tana tuānga ki te whakahaere i te whakapapa kiko

Te taipitopito me te roanga o te Biotherapy i te Dermatology

Ko te mātai arataki e mahi ana mā te whakamahi i ngā pūrere mai i ngā arataki ora pērā i ngā huaota, ngā tekau hōhū, me ērā atu wāhanga iti o ngā selu e kiia nei ko ngā exosome hei whai muka i ngā raru rokohiko roa pērā i te eczema, te psoriasis, me ngā tohu o te wherā. Ko ngā mātai tawhito ka huna noa i te raru, engari ko te mātai arataki ka eke atu ki te take matua o ētahi nui o ngā mate kiko - aronga ki te ara tūmatanui o te tinana. I tētahi rangahau i te tau 2022 i te pukapuka whaiaronga Frontiers in Bioengineering and Biotechnology i whakaatu i tētahi mea hoki: i te mātai i te psoriasis e te aitautoimmune e hāpai ana, i iti ake te hoki mai o te mate (ko te toru haurua) i te mātai arataki mā te steroid e ai ki te mahi a Bellei i te tau ki muri. E tohu ana tēnei he māmā pea te mātai arataki ki te whai wāhi roa ake i te whakahaere i ēnei aronga, kaua ko te whakamauritanga poto anake.

Te Tūwāhi o ngā Hua Arataki ki te Whakahaere i te Tūmatanui o te Kiri

Ko ngā huinga mataora e whakamahia ana i te maimoatanga mataora e mahi ana ki ngā ara matua o te punaha ārai mate mā te pupuri i te taurite o ngā taumata cytokine (pērā i te TNF-alpha, IL-6, me IL-10) pērā anō hoki te whai waahi ki te arataki i te whanonga o ngā makropāhī (arataki ai pea rātou hei M1, pea rānei hei M2). Mā te whiwhi i ngā exosome mai i ngā sel mitotic mesenchymal, e utu nei ngā karuwhenua iti nei i ngā microRNA e here ana i te ara tohatoha NF-kappa B, e pakari ake ana i ngā wā o ngā pānga ekzema. I whakaatu ngā rangahau tēnei kaupapa e huritau ake ana te wā maimoatanga i te aukume kiko 21 ōrau tetai ake i ngā maimoatanga tuarangi pērā i ngā herehere calcineurin e ai ki ngā rangahau a Wang me ana hoa i te tau 2019.

Te Hononga I Waenganui I Te Maimoatanga Whakarewa Me Te Ārai Kiri

Ko te maimai hauora whakaora i ngā tikanga tūmatanui mā te whakauru i ngā sel mitipua e hangaia ana e te tinana. Kāore i te whakamāmātia noa te pānga kino, engari ko ngā whakaritenga mai i ngā sel mitipua mesenchymal e āwhina ana hoki i te hanga i te collagen hou me te whakarerekē i ngā whatukuhā kua pakaru mā te tohatoha karapoti ki waenganui i ngā sel. I ētahi rangahau hōu, i kitea ai e te timatanga a Paganelli i te tau 2020, ko te hononga o ēnei whakaritenga sel me ētahi matapae hoahoa hei whakatikatika i ngā hanganga biologik he pai ake te whakaora i ngā ririhau ki te 40%. Ko tēnei takotoranga e āwhina ana i te mātāpuna kia pakari ake te herekave i ngā mate urutā, me te whakaora anō i muri i te ririhau.

Ngā take bio-mātai me te whakawhitinga kōrero ki waenganui i ngā vesicles waho-sel

Ngā exosome ki te tu maturity me te tu mātātoko: Ngā tikanga o te tohatoha kōrero ki waenganui i ngā sel

Ko ēnei pātiti iti o ngā exosome e mahi ana pērā me ngā kaituhi i waenganui i ngā selu, i te reanga noa hoki, i te reanga e pakaru ai hoki te kiri i te rā. E kawe ana ratou i ngā mea katoa pērā i ngā huaota, ngā mātukutuku, me ēnei tapuwae iti RNA hei āwhina i te whakatika i te taumata selu. I ētahi rangahau hōhonu ake mai i te Journal of Nanobiotechnology i te 2023 ka kitea ko ngā exosome nei ka āwhina i te whawhai ki te tohatoha oksikara mā te kawe i ngā eneima catalase. I whakaatu ngā tūhuratanga ko te maimoatanga UV i ngā selu kiri tangata i te 40 ōrau. Anō hoki, kātahi ka tukuna e ēnei exosome anoo ngā tohu TGF-beta e ara ana i ngā tikanga hanga collagen ki roto i te kiri. Ka āwhina tēnei ki te whawhai ki te pakaru o ngā whatunga tautoko pūnoa e āhua tūpono ana ake kia matua.

Ngā Exosome nō ngā Selu Matua me tō rātou Uara Huarahi ki te Whakahaere Mōhio

Ko ngā exosome ā MSC e tuku ana i te whakahaere mōhio kia rua ngā wā: tuatahi he whakamāu i te pānga hauhake ki te tuku IL-10, kātahi ka whakapakari i te repair o te kiri trūwhara Māori rerekētanga. I ngā tauira clinician, ko ēnei mouta exosome e hoki tonu ai te arotake kiri 63% tere ake i ngā serum huarahi tipu, nō reira he mea tino pai mō ngā tikanga biotherapy e whai ana ki te aukati i te mōhio kore.

Ngā Sel MSC (Mesenchymal Stem Cells) Me Ō Rātou Tāwāhi Paracrine Ki Te Repair Kiri

Ko ngā selu matua e whakarato ana i ēnei pūhui iti, i te mōhiotia hei wēhanga o waho e kī ana ki te tēnei rau rānei atu kikokiko wehe kē. Ko ētahi o ēnei ko ngā kāpo whakahaere TIMP e taua ana ai ngā eneima MMP kia kaua ai e waro ngā huinga korekeri. Ina whakaputaina ki te kiri i pau ai e te rā, ka piki ake te hua o te korekeri momo I mai i te 29% i muri i ngā wiki e waru. I te wā kotahi, i te 41% te heke iho i ngā taumata TNF alpha. He aha ngā hua? Ko te whakaora anō o te kiri e rite ana ki te mea ka whakaputaina ngā selu matua tika, engari kāore ngā aronga e tino taea ana e te mātai selu.

Ngā Matēriera Biomatāiaki E Hāpai Ana i te Tuku Wēhanga O Waho

Kāwhai anō ngā kaitiaki karawhiti hauhotaka i te noho tūroa o ngā pūpahi waho wāhi ki te 300%, e whakarere ana i ngā utu whakarewa mō ngā utu whakahou. I whakaatu tētahi punaha tauira karawhiti i te 2023 i te 82% ake te whiwhinga fibroblast ki ngā takotoranga roroimata pōroiroi tērā i ngā EV māma, ka hono tautoko hanganga me te tuku kargo āhuatanga ki te whakapakari i te whakahoutanga arataki matatoko.

Ngā Pānga Whakarereketanga Arataki o ngā Momo Kiko ki ngā Tūrua Matatoko Huringa

Te Māmātanga Hihi me te Huringa ki te Tau e Mātau Ana: Te Whiwhinga Mā te Biotherapy

Ina whekua ngā sel mitaiao i te mate piko kiora, ka hinga tō rātou āhua matatoa ki te noho tika. Kua kitea e mātou ngā akirira e whakaatu ana koirā anō te pānga o te ao huaoro ka taea te piki ake i ngā taumata o ngā momo huaoro (ROS) mai i te 38% e ai ki ngā rangahau i tukuna ki Frontiers in Medicine i te tau ki muri. Ko konei te wāhi e uru mai ana te maimai aronga, arā anō pea te tuku i ngā eneima antiokihana pērā i te superoxide dismutase ki te pakanga ki ēnei molekiū hekei ROS. Kāore anō hoki ngā eneima e tuanui ana, engari anō hoki ka mahi hei whakarere i ngā tohu NF-kappa B e āhua nui ai te mate. Ko te mea e pai ai tēnei ara ko te āwhina i ngā mitochondria kia rere tika, me te iti iho te peroxidation o ngā hiako umu ki te toru haurua. Te putanga? Ko te kiri e noho hauora ake ana i te roanga atu, nā te mea kāore i te wehe tonu te collagen, ā, kāore hoki ngā takotoranga o waho e iti haere ana i ngā mōrearea rāwhiti.

Te Whakahaerenga o Cytokine (pērā ki te TNF-α, IL-6, IL-10) mā ngā Bio-factors

Ka whakaturia e ngā hūmata bio-ngota ngā rerekētanga o te kōtiritiri i ngā mate māhu o te kiri pērā i te psoriasis. I tētahi rangahau 2023 ka kitea ko ngā exosome mai i ngā tikanga taiao e penapena ana i ngā TNF-α, IL-6 hāpai māhu kia 52% ake, i te wā e hāpai hoki ana i te IL-10 anti-māhu. Ko tēnei whakarāranga māwhitiwhiti e whakatika ana i ngā pouri ngota kāore i te whakamātau whānui, he whakaurunga haumaru ake i ngā taputapu hangarua mā te whai wa hirotanga ki te wehenga o ngā sel M17Th.

Te Whakawhitinga Makrofaji (M1/M2) i te Whakaora Tahumaero me tōna Whakarereketanga e te Biotherapī

Ka hāpai te biotherapī i te neke atu mai i te M1 hāpai māhu ki te M2 hou hou trū mā te tohatoha vesicle ā-waho. Ka whakapai ake ngā matapae MSC hua-koiora i ngā ropū M2 mā te 81% (ScienceDirect, 2023), ka whakapai ake i te whakaatu tahumaero mā te tuku TGF-β1 me VEGF. Ka pai ake tēnei hurihanga i te kapatanga paru mā te 40% i ngā paru roa, ā, ka heke te mōrearea o te fibrosis e hāngai ana ki te mahi roa o M1.

Ngā Hūmata Tipu me ngā Ara Whakaora Kiri Ā-Tinana

Ngā Hūmata Tipu Matua me ngā Ngota Kōtiritiri i te Whakaora ā-Biotherapī

Kei te tīmata tonu te ao o te maimoatanga bio ki ngā molekua tohu motuhake pēnei i te EGF me te TGF-beta e āwhina ana i ngā tikanga whakaora i te kiri. Hei tauira ko te EGF e tīmata ana i te hiki ake i ngā sel keratinocyte me ngā fibroblasts mai i te 40% ina whakaoratia ngā whatianga, ā, e ai ki ētahi rangahau i tūhonoa ai i te tau ki muri ki te Frontiers in Cell and Developmental Biology. Ko TGF-beta ka mahi anō pea ia rerekē, e pupuri ana i ngā tohu pāpāhua pērā i te IL-6 i te wā e āwhina ana i te hanga anō i te mātai extracellular. Ā, āniania nei kei te kitea ētahi whanaketanga hou hoki ki ēnei matapae biomimetic hou kei te mākeke. Mā rātou e tiaki ngā faktoro tipu atu pērā i te FGF-7, i whakatupeora ai he toronga elastin e te 34% nekehia, ina hua ai te kiri i ngā tau o te tūāpapa. He mea ataahua rawa tēnei mōku.

Te Whakauru i te Whakaora Kiri Anawhenua Trūnga Ngā Hua Bio

Ko ngā whanaketanga hou ki te maimoaioitini e hoki ana i ngā tikanga mātaiarai tūmatanui o te kiri, e taraiwa ana ki te whakatika i te mate mitochondria me te wehe atu i ērā kaipara hauhake hauwhenua e pāngia ai te raru. Hei tauira ko ngā exosome turawhiti-to-fibroblast, ā, ēnei karuwhitu itiiti e āki ai i te ara Nrf2 anti-oxidant, i whakaatu ai hoki i te heke haere o ngā tohu mate oksikatiaki umanga 60% i ngā tauawhi kiri tuimaro. Ko te mea e nui ake nei te aronga ki tēnei maimoaioitini ko te ara e whakaora anō ai i ngā sel miti a-tinana, ā, e āwhina hoki i te hanga anō i ngā papa takotoria e pakaru ana trūnga te whakahaere pai ake i ngā rahi MMP-1, me te āki i te hanga tika o te collagen III. I kite hoki ngā kairangahau i ētahi ara hihiri hei tuku i ēnei maimoaioitini. Ka tuanui ake ngā hydrogel thermoresponsive, nā te mea ka piki ake te wātea mai o te matū ki te 66% tata nei i ngā serum noa i raro i ngā rangahau hou i tāngia i te tau ki muri. Ko te whakapai ake i te tukunga, kia māma ai te ora, kāore he totohu māmā, ā, ānoa i ngā whati pumau e pēhia ana e ngā maimoaioitini tūmatanui.

Ngā Tōpana Taiwhenua ki te Biotherapy mō te Whakawhitinga o te Hautētika

Ngā aronga hou ki te inihua o ngā papa tūpui me ngā āhuatanga bio-hāngai

Kei te whai hua ngā kairangahau i te pānui mātātoko 3D i ngā hanganga kiko e tu ana ko ngā huinga matatoko noa mai i ngā ratake anō, e āwhina ai i te whakahaere ake i ō rātou puna humarongo tika ake. E ai ki tētahi rangahau hou e pā ana ki ngā Mātai Hauora Matua i te tau ki muri, i whakaatu te takotoranga o ngā paraihe motuhake e kiia nei he bioactive hydrogels me tētahi mea e kiia nei he phage therapy kia huritau te whakaora i ngā whati kua matepukupuku e te 35%. He mea ataahua rawa! Kei te tīmata hoki ngā taputapu pūkenga tangata ahumāni ki te urupare ki konei, e āwhina ana i ngā kairangahau kia mārama ai ki te takotoranga pai rawa atu o ēnei huinga matatoko mō ngā puna humarongo o ia tangata, ina nui ake mō ērā e pāngia ana e ngā aronga pērā i te psoriasis, i te lupus kei hea ka pāwhara te tinana ki a ia anō. E fit ana katoa tēnei ki te kōrero a ngā tohunga i tētahi pepa mai i ngā Ara Tirohanga mō te Bioengineering me te Biotechnology i te rautau nei, i kī ai rātou me pai ake i te hanga i ētahi putanga ritenga o ēnei karuwha ā-temepara iti e kiia nei he extracellular vesicles.

Ngā whakapākanga huinga kawemai me ngā ara rāwekenga

Ahakoa ngā putanga pai i ngā tūhuratanga mua-iwi, kāore anō kia neke atu i te 12% o ngā maimoatanga mō ngā hēkinatanga matihiko i whiwhi tono ki ngā tūhuratanga Phase III i waenganui i 2020–2023 nā ngā raruraru paerewa. E hiahiatia ana e ngā rāwekenga herehere ngā tūhuratanga whai hua o ngā wae pito o ngā exosome, āhuareka anō ki te kai tuku cytokine. Ko ngā rerekētanga herehere i waenganui i ngā motu e areri ana i ngā whakaaetanga, ka tohua ai te hiahia ki ngā tūtohi kotahi hei whakarite i te haumāta, i te wā e huritau ana te whakauru ki te maakete.

Ngā punaha tukatuka smart e whāinga ana ki te pangahau o ngā macrophage

Ko ngā tauranga biomimetic hou e whakauru ana i ngā punaha whakapaunga arataki e āwhina ana i te huri i ngā makaporahi mai i tō rātou āhua M1 māmā ki te momo M2 whakaora. I tētahi mahi tuatahi, i titiro rātou ki te whakamahi i ngā exosome mai i ngā keratinocyte ki roto i ēnei kīrepo taraiwa āhuatanga pH, ā, i kitea e ngā kairangahau he paku ake te wā (pera me te haurua) o te māmā tonutanga roa i ngā tauira weu ki te upoko, mōhio atu ki ngā tikanga tawhito. I reira hoki tētahi pepa tino hihiri i tūhuratia tau kua pahure ake i APL Materials e whakaatu ana i te pehea o te tāpiri i te graphene oxide ki ēnei taputapu iti māmae ka pai ake te tae atu ki ngā wāhi tika o ngā sel immune kiko ma te 72 miriona tōrahi ake i mua. Ko te whai hua o tēnei whai waahi pai ake ko te iti ake o ngā urupare immune kore e hiahiatia ana ki ētahi atu wāhi o te tinana, he mea nui tēnei mō ngā whakamahinga ā-tiriti.

FAQ

He aha te biotherapy i te dermatology?
Ko te biotherapy i te dermatology e whakamahi ana i ngā mea matatini, pērā i ngā protein me ngā exosome, hei whakatika i ngā raru kiko pērā i te eczema me te psoriasis mā te whai i ngā herenga herenga e here ana i te punaha immune.

Me pēhea e whakarāwekenga ngā hua taunahatanga ki te mōhiotia o te kiri?
Ko ngā hua taunahatanga e āwhina ana i te taurite o ngā cytokine me te whai wāhi ki te noho rautaki a te makropūra, nā reira ka pai ake te mōhiotia o te kiri, ā, ka tere ake te hoki kia ora.

He aha te tūranga o ngā exosome ki te tiaki kiri?
Ko ngā exosome hei karuwhero e uta ana i ngā protein me ngā waahanga RNA i waenganui i ngā sel, ā, e āwhina ana i te whakaora i te kiri me te heke i te totohe oksikihana.

He aha ngā wero e āhua ana ngā maimoatanga biotherapy i te whakamaarama kliniki?
Ko ngā wero ko te whakaritenga i ngā maimoatanga bio-factor me te arataki i ngā ara herehere, e rīwai ana i te whakamahinga whānui.